Wako-Bambanker无血清细胞冻存液，无需程序降温，-80℃长期保存！

关于WAKO

英文全名：Wako Pure Chemical Industries, Ltd.，中文全名：日本和光纯药工业株式会社，成立于1922年，是全球一流的试剂制造厂商，以其高品质的试剂闻名世界。成立九十多年一直致力于高质量的试剂及药业的生产与开发，所生产的各类产品均获得ISO9002 认可。“Wako”系列试剂涵盖了全面的生物化学、分析化学、有机化学、环境分析、食品.医药品分析、高纯度及认证标准品等多类，约有50万种试剂。

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传统的细胞冻存遵循缓冻速融原则，细胞+DMSO+血清，然后-4度30分钟—— -20度1-2小时—— -80度过夜——沉入液氮，或者使用程序降温盒。

而Wako旗下两款无血清即用型细胞冻存液，无需程序性降温，可在-80℃长期保存细胞。节省血清、节省购买程序降温和液氮所需的昂贵费用，最关键的可以节省您的宝贵实验时间！

● BambankerTM无血清细胞冻存液（收集细胞，加入产品后-80℃长期保存或-80℃过夜后置于液氮中保存）

   ● BambankerTM Direct 无血清培养基（无需离心收集细胞）

1、BambankerTM无血清细胞冻存液

产品特点     

       * 无需程序性降温，直接使用

       * 可-80℃或液氮中长期冻存

      * 即用型细胞冻存液    

       * 无血清，成份确定，避免由支原体、病毒、朊病毒及其他病毒颗粒引发的污染

       * 产品经无菌检测，符合药典

      * 适用于肿瘤细胞和常规细胞，有研究表明同样适合各类敏感细胞，如ES细胞、原代细胞

       * 高复苏率（请见对比图效果）   

操作流程：

①收集对数生长期的细胞（5 x 105 ~ 1 x 107）；

②用1ml BambankerTM重悬细胞，置于冻存管中，无需预冷，直接-80℃或液氮中长期冻存。

③用恒温箱或者水浴锅快速复苏细胞。

   * 细胞必须处于对数生长期（建议对数期，复苏率会更高）

操作流程图示

无菌检测：

内毒素：生色底物法

支原体：荧光抗体法

真菌和细菌：依据药典

（可索取无菌检测检验证书）

BambankerTM 与其他相关产品比较：

应用

【低温冻存实验证明以下细胞保存完好】

　　ES细胞（小鼠）使用实例。
　　T.Hikichi,et al; Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 2007, 25, 46-53

2、BambankerTM Direct 无血清培养基（无需离心收集细胞）

产品特点：

本品不需要复杂的离心步骤收集细胞，只需要往养有细胞的培养基内加入等量的本产品，再分装到冻存管，放入-80℃或液氮中长期冻存。

①不需冻存前预处理，操作简便；   ②不需稀释直接使用； ③无需分步降温，直接使用；

④可快速、长期冻存细胞（-80℃或液氮）                 ⑤不含血清

流程图示：

BambankerTM参考文献（部分）

Bambanker Publications   List

00001. Hikichi T., et al.: Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 25: 46-53 (2007).

00002. Thuy BH, et al.: Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development. Reproduction, 141: 67-77 (2011).

00003. Saeki K. et al.: High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells, Cloning and Stem Cells, 4: 509-22 (2009).

00004. Takata Y. et al.: Generation of iPS cells using a BacMam multigene expression system. Cell Struct Funct., 36(2): 209-222 (2011)

00005. Zaidi S. et al.: Runx2 deficiency and defective subnuclear targeting by pass senescence to promote immortalization and tumorigenic potential. PNAS., 104(50): 19861-19866 (2007)

00006. Miono S., et al.: Characteristics and Function of Cryopreserved Bone Marrow-Derived Endothelial Progenitor Cells. Annals of Thoracic Surgery., 85: 1361-6 (2008)

00007. Hatoya S., et al.: Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes, Journal of Theriogenology,66: 1083-1090(2006)

00008. Shimizu Y. et al.: Impaired Tax-specific T-cell responses with insufficient control of HTLV-1 in a subgroup of individuals at asymptomatic and smoldering stages: Cancer Sci., 100 (3): 481-9 (2009)

00009. Sato D., et al.,: Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse therapy in IgA nephropathy patients.,Nephrology Dialysis Transplantation, (2011)

T-Cell

00001. Park HS, et al.: Bone marrow T Cells are superior to splenic T cells to induce chimeric conversion after non-myeloablative bone marrow transplantation, Korean J. Intern Med., 24 (3): 252-262 (2009)

00002. Huang YH, et al.: Dental Stem Cells and Tooth Banking for Regenerative Medicine, Journal of Experimental & Clinical Medicine, 2 (3): 111-117 (2010)

00003. Lee SY, et al.: Effects of Cryopreservation of Intact Teeth on the Isolated Dental Pulp Stem Cells, Journal of Endodontics, 36 (8): 1336-1340 (2010)

00004. Huang MS, et al., Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp.Journal of Dental Sciences, 6(1): 48-52 (2011))

00005. Agata H., et al., Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells,Differentiation, 76 (9): 981-93 (2008)

00006. Kamada H., et al., In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field, American Journal of Orthodontics & Dentofacial Orthopedics, 140 (6): 799-805 (2011)

00007. Mieno S., et al., Effects of diabetes mellitus on VEGF-induced proliferation response in bone marrow derived endothelial progenitor cells. J. Card Surg., 25 (5): 618-25 (2010)

00008. Oshima N., et al., Optimized method for culturing outgrowth endothelial progenitor cells, inflammation and Regeneration, 31 (2): 219-227 (2011)

0009. Liu DG et al, Relation between human decay-accelerating factor (hDAF) expression in pig cells and inhibition of human serum anti-pig cytotoxicity: value of highly expressed hDAF for xenotransplantation, Xenotransplantation, (1): 67-73 (2007)